Table 1 Primary antibodies used in this study

HMB45

1: 200

Ventana CC1 100 °C

Milipore, Temecula, US

Melan-A (Mart-1)

1: 1000

Ventana CC1 100 °C

Cell Marque, Rocklin, US

D2–40

1: 50

Ventana CC1 100 °C

DAKO, Glostrup, Denmark

Phosphorylated mTOR

1: 100

Ventana CC1 100 °C

Cell signaling, Boston, US

C-Kit

1: 500

Ventana CC1 100 °C

Abcam, Cambridge, UK

SMA

1: 500

Ventana CC1 100 °C

DAKO, Glostrup, Denmark

CD34

1: 200

Ventaan CC1 100 °C

DAKO, Glostrup, Denmark

Caldesmon

1: 1000

Ventaan CC1 100 °C

DAKO, Glostrup, Denmark

Desmin

1: 200

Ventaan CC1 100 °C

DAKO, Glostrup, Denmark

Myogenin

1: 500

Ventaan CC1 100 °C

DAKO, Glostrup, Denmark

Ki-67

1: 100

Ventana CC1 100 °C

DAKO, Glostrup, Denmark