Fig. 2
From: Establishment of multiplex RT-PCR to detect fusion genes for the diagnosis of Ewing sarcoma
Specificity of multiplex primers to each fusion gene. Multiplex PCR for EWSR1/FUS-ETS transcript variants using Set A (a) and Set B (b) primers. Lane M: Trackit 100-bp ladder marker (a) or Trackit 1-kbp plus ladder (b). Molecular sizes are indicated in the right with yellow arrowheads (a) and red arrowheads (b), respectively.; lane 1: NCR-EW2 cDNA (EWSR1-FLI1); lane 2: WES cDNA (EWSR1-ERG); lane 3: EWSR1-ETV1 plasmid; lane 4: NCR-EW3 cDNA (EWSR1-ETV4); lane 5: EWSR1-FEV plasmid; lane 6: FUS-ERG plasmid; lane 7: FUS-FEV plasmid; lane 8: HEK293 cDNA; lane 9: no template control. The plasmid samples contained the same amount of HEK293 cDNA as the Ewing cell lines