Fig. 2

Flow cytometry analysis of peripheral blood. Through a wide monoclonal antibody panel by 8-color flow cytometry analysis, two distinct blast populations were detected (P3). One population occupied 40.3% of all nucleated cells (red), with strong expression of CD38, CD36, CD2, partial expression of CD41, CD61, CD42b, CD34, CD117, CD13, CD123, CD56, CD7, dim expression of HLA-DR, CD33 and absent expression of CD19, CD10, cCD79a, cCD3, CD5, TdT, MPO, CD203c, CD235a. The other population constituted 40.50% of all nucleated cells (purple) and displayed as CD7bri⁺ CD38bri⁺ CD2⁺ CD34⁺ CD117⁺ HLA-DR⁺ CD33⁺ CD11b⁺ CD123⁺ CD56⁺ cCD3^par+ CD13 ^par+ CD19 ^par+ and CD5⁻CD36⁻TdT⁻MPO⁻cCD79a⁻CD19⁻CD10⁻CD203c⁻CD235a⁻CD41⁻CD61⁻CD42b⁻.