Fig. 8

Detection of NRG1, immune cells influenced by NRG1, and PI3K/AKT in clinical tissue samples. A IHC detection of S100 expression in clinical tissue samples. con: normal vestibular tissue. S100 is mainly expressed in the BNST cytoplasm. S100 is a biological marker of BNST—scale bar: 20 μm. The statistical graph is on the right. B RT-qPCR detection of Nrg1 mRNA expression levels in clinical tissue samples. C Western blot detection of NRG1 and key proteins in the PI3K/AKT pathway in clinical tissue samples. The statistical graph is on the right. D IHC detection of NRG1 expression in clinical tissue samples. Mainly distributed in the cytoplasm and membrane of myelin-forming cells and macrophages. Scale bar: 20 μm. The statistical graph is on the right. E Changes in M2 macrophages (M2 mac) and neutrophils (neu) in clinical samples detected by IHC. CD206-scale bar: 20 μm. CD66b: scale bar: 20 μm. Both are expressed on the cell membrane of macrophages and neutrophils. Statistical graphs on the right. F Changes in M2 macrophages and neutrophils in clinical samples detected by flow cytometry. M2 mac: CD68 + CD206 + , neu: CD14 + CD66b + . Statistical graph in the first quadrant. con group: n  = 3; BNST group: n  = 5. Indicates a difference compared to con, where P  < 0.05 indicates a difference compared to con, P  < 0.01, and *** indicates a difference compared to con, P  < 0.0001